Tuesday, August 25, 2020

Living in a big city or living in the contryside Essay Example for Free

Living in a major city or living in the contryside Essay It has for quite some time been a debate among individuals that living in a modest community or in a major city is progressively best. While a few people guarantee that living in such huge urban areas as capital or some other extraordinary city can bring about progressively agreeable, others feel that habitation in the littler town can't just give them quiet lives yet additionally make them more advantageous than individuals living in a major city. As I would see it, I have preference for living arrangement in a humble community because of certain reasons. In spite of the fact that town-occupants can endure significantly more helpful than the individuals who live in the wide open having numerous offices and enhancements for them, they should defy with much contamination in their old neighborhood. That little particles from enormous ventures and harmful gases from million vehicles will in general increment increasingly more are utilized to portray the town these days. In actuality, ailing ahead of time advancements makes country life upbeat and serene. Rustic occupant can appreciate living in genuine nature. They can burn through Just a brief period to go to work, not at all like town-tenants who experience a lot of difficulty with road turned parking lot and lso they can spend times they left during the day to accomplish progressively agreeable and more clever exercises, for example, exercise, shopping or playing game with their kids. In addition, individuals who live in the field are probably going to have longer life span and more beneficial than the individuals who possess in the town. High advances can damage and cause so genuine medical issues to the individuals who are inhabitant of the town. All in all, I would prefer to live in the little nation than in the enormous city. Despite the fact that, the solace capacity in the town offers numerous individuals, I like to go through my time on earth basically and cheerfully.

Saturday, August 22, 2020

Radio-frequency identification Essays

Radio-recurrence distinguishing proof Essays Radio-recurrence distinguishing proof Essay Radio-recurrence distinguishing proof Essay Capital Safety Is the worldwide pioneer of fall insurance, limited space and salvage hardware. RIFF (radio recurrence Identification) Is a remote information assortment innovation. RIFF innovation can Identify, follow and appoint Information to one of a kind things without direct contact or view examining. I-Safe TM comprises of uninvolved RIFF labels (transponders) that can be examined by versatile perusers (recipients) that synchronize information to an electronic secure data entrance. Theoretical 1. The current task being examined is on how radio recurrence distinguishing proof yester (RIFF) has been executed to assist better with overseeing capital wellbeing fall security gear. The data framework being utilized has become an Important job on how they deal with their item to help, cost, Improve wellbeing consistence, lessen hazard, and upgrade security inside government guidelines. Capital security has become the worldwide pioneer In fall assurance. With the utilization of radio r ecurrence Identification gadget they can tract and malting their hardware In auspicious way. 1. 2 Author and Team Member Group Project Assignment #5 Mohamed, Lei Thanksgiving Travis Boyd, Each Jones Carl Basilica, Young Yang Table of substance 1. 3 Target of study 1. 4 Capital wellbeing Is the association being concentrated In our gathering. They can be distinguished by their NAZIS and SIC codes that put them In Industries like: oil and Gas, development, transportation, utilities, wind vitality, general and private fall insurance. They are the worldwide pioneer In assembling fall security for these different organizations that are needing wellbeing assurance that are working over the ground and underneath with their restricted space gear . Capital wellbeing deals are Latin America and Australia Capital Safety must be efficient. That is the reason they have embraced the radio recurrence recognizable proof gadget to help better-deal with their items. Capital Safety is the primary organization to execute radio recurrence ID framework on their fall assurance gear. At the point when they presented their product I-safe 2. 0 and afterward made improvement to I-safe 3. 0 to help examine these RIFF labels. The I-safes. O helps track, support and review when terminated. With the utilization of radio recurrence distinguishing proof framework, improvement to their organization implied endeavoring o be the best. The radio recurrence ID gadget decreased cost, time, wellbeing and diminishing danger has made it progressively controllable The data framework radio recurrence ID gadget RIFF is innovation that is effectively accessible in showcase. Capital wellbeing anyway utilizes its own specially crafted innovation created in - house. The business procedure of the framework is that every item accompanies the round gadgets incorporated with the item. The business procedure of the framework is that every item accompanies the roundabout gadget incorporated with the item. The items are filtered at the processing plant by the assessor that did the last investigation or individual who concluded the gadgets. Every item is relegated and ID number to the framework. The framework can record and tally the items naturally, and alert the organization when the items are expected for review. Utilization of RIFF Has expanded quickly and would now be able to be found in PDA, government IDS, Passport, a few guns, bundles dealt with by the postal framework Etc. Capital security is one of the main organizations to execute this innovation to their fall assurance gear. By speeding up and the effectiveness in their security and distribution center zones capital wellbeing has figured out how to get progressively beneficial as well as give better client support by keeping up legitimate hands on administration and giving auspicious review when required. Capital Safety has made their organization increasingly gainful with the utilization of radio recurrence gadget. With the utilization of radio recurrence distinguishing proof gadget this data framework has become a key factor on how they use this administration device to help sort out their organization structure with their item. Official rundown 1. 2 It is enthusiastically prescribed for different organizations to use this kind of data framework to help with the association of their organization item. I realize that radio recurrence distinguishing proof gadget is a valuable apparatus that could be utilized in various parts of business association. It tends to be utilized in various parts of business, climate the utilization of ID labels for representatives, sequential construction system workstation, following parts, and stock. Emerson process is another organization that has used this kind of data with their business procedure. They have radio earnestness ID labels for their workers for access to and out of the structure. They have related this kind of data alongside their get together workstation. They utilize radio recurrence ID gadget to sign all through every workstation. That way they know whether there is an issue they can tract which worker is related with that specific get together. For a considerable length of time RIFF gadgets have been utilized in a wide range of territories (Clayton Boyce, 2003). One such territory has been as an enemy of robbery gadget. Most shoppers have bought items that have held RIFF gadgets. Stores at the nearby shopping centers have been utilizing hard plastic label against burglary gadgets on stock for in any event she realizes it's anything but a smart thought to stroll to near the passage way. Numerous supporters have endured the shame of strolling around a garments rack close to the front entryway with things to be bought and hearing an ear parting sound. This was brought about by the RIFF gadget on the apparel coming into contact with the electromagnetic field at the entryway. Other over a significant time span utilizes for RIFF include: programmed fee collection counter passes, creature following chips, and friends get to identifications.

Wednesday, July 29, 2020

The Unspoken Rules of Calling in Sick

The Unspoken Rules of Calling in Sick You wake up one morning with a sore throat and you realize that although that pending deadline needs to reach your boss’s desk by the end of the day, you have no energy to get out of bed.So, what do you do?You proceed to your contact list and begin your preparations to call in sick.After all, it’s better to take a day or two off rather than prolong the flu, right? While calling in sick is a sensible procedure, you need to take into consideration whether your boss and co-workers are going to be fine with your absence.One-third of U.S. employees have faked their illness and hence, it’s necessary to come up with a good “stay home” strategy to ensure you don’t step on anyone toes.ARE YOU REALLY SICK OR IS IT JUST A BAD MONDAY?First things first! Get out of bed and locate the trusty thermometer that you just can’t seem to find when you are under the weather.Recording your body temperature is one of the quickest ways to finding out if you are indeed sick and it’s not just a ll in your head. Once you’ve noticed a higher temperature than normal, it’s time to put things into perspective.A running nose, sniffles, or a cough is often the first tell-tale signs that you are going to be sick. It’s important to never mistake a seasonal allergy to the actual flu.While the flu is contagious and it’s often best to rest at home rather than to infect everyone in your office, a seasonal allergy isn’t worth taking a sick day and once the usual body ache begins to subside, you will start to feel better.According to CareerBuilder’s Annual survey, in 2017 a staggering 40-percent of workers have called in sick when they have been in the pink of health. Among the many excuses given by the participants, here are the top dubious reasons for calling in sick by the report â€"Called in sick for having too much foodTook the day off for believing themselves to be deathly illExperiencing traumatic stress and panic attacksFelt under the weather for visiting a funeralUna ble to find their car keysIt comes as no surprise as to why your boss could suspect you’re lying when you are indeed sick with the kind of excuses that are listed above.When calling in sick always ensure you have a valid reason and relevant proof, like medical documents, to back up your claims post your sick day period.This is to build your credibility and trustworthiness with your colleagues and boss.Visiting the emergency room or scheduling a doctor’s visit should be among your top priorities when calling in sick.If you truly believe you’ve caught a contagious disease, a medical expert can help you get over your illness at a faster pace by prescribing the necessary medication. You’ve also got a doctor’s note to prove your leave of absence and your boss will have a much better time believing your sick tale.This interactive video shows how to get well soon which means significantly fewer days spent at home from work. 5 RULES TO KEEP IN MIND WHEN CALLING IN SICK1. Check wit h Your Team FirstIf there’s an important project on the line, it’s necessary to check the reactions of your team.If you’ve managed to trigger a response of caution from them, it may be alright to call in sick but if they find themselves panicking over the project situation, you may need more coaxing to ensure things work out fine in the end.Explain your situation in quick and easy conversations such as â€"Example 1“Hello Mark, I hope the day’s found you in good health, unfortunately, the same can’t be said for me. If it isn’t too much to ask, can you please fill me up on the day’s proceedings and redirect my visiting clients back to my office number. Thanks for taking the trouble and I hope to be back at my physical and mentalpeak.” Example 2“Hey Nancy, I won’t be able to come to work for a couple of days as I am feeling under the weather. Do share the news with the rest of the team and let them know that I truly regret the decision. Please do share the day’s discussion with me post your work hours so I can update myself on the project’s status. Thanks, wishing you the best!”Your colleagues will legitimately feel sorry for your condition and sympathize with you when you can provide them with a valid reason.Keep in mind that it’s considered a professional courtesy to update your colleagues that you aren’t showing up for work at the start of the day to ensure they plan out a strategy when dealing with your absence.Your co-workers are bound to feel annoyed when they come to know of your absence without any intimation from your end.Seeker demonstrates the reasons why shouldn’t go to work when you’re in this informative video. 2. Focus on Getting WellThere is no way to know when you’re going to fall sick or to calculate exactly how long the flu would last.However, you could take the necessary precautions of minimizing the days you’re in bed by ensuring you get treated for your condition at the earliest. Eliminate the stress f actor and focus on getting as much rest as you can.Here are a few short tips to get you back on track â€"Get More Sleepy TimeIt goes without saying that when you’re sick the fastest way to the road to recovery is to get some quality shut-eye.Depriving your body of sleep can cause strain to your body and in the long run, you extend your leave of absence.Consume Protein-rich FoodsProtein provides our body with muscle recovery aid and is generally a great way to gain nourishment when sick.Often, a sick person can’t consume a lot of carbohydrates due to their appetite taking a hit, that’s when a light protein snack can do wonders for your body.Drink Plenty of WaterWhen ill, your body tends to dehydrate a lot and a loss of water can inhibit your ability to heal from the sickness.H2O is often the best way to beat an illness as hydrating yourself often replenishes lost fluids and is a quick way to get back to your prime shape.Listen to Soothing MusicMusic offers a gateway to the mind to get away from the constant stress of everyday work life.Similarly, when you’re sick it’s a great way to pass your time without stressing your eyes by watching television or spending long hours on the computer. Setup a great playlist or a podcast and gently rest your eyes.Wear Loose Clothing and Try Over the Counter MedicationIf it’s a high fever you’re better off getting yourself checked at the doctor before self-medicating.On the other hand, if you’ve contracted a common cold, wearing loose clothing can help disperse body heat better and make you comfortable and anti-inflammatories such as aspirin and ibuprofen are recommended.When you do get back to your office, it’s important to practice safety measures to prevent infecting others. Rob Donofrio, a microbiologist at NSF International suggests “Proper handwashing with soap and warm water is still, by far, the best way to prevent the spread of germs and bacteria. Similarly, disinfecting common areas like the office kitchen or eating area, copy machine and printer can be crucial to keeping germs at bay.”3. Handover your Duties to a Trusted Co-workerUndoubtedly, every employee is unique to the company and there are tasks that only they can complete as they understand the procedure and the basic operation of the project.Sickness, on the other hand, doesn’t come with a warning and hence, you are bound to fall ill during the busiest time of the year. In these cases, it’s always convenient to have a fellow colleague, that you can trust to lead the way in your absence.It’s important to follow up with your colleague by either â€" phone or email.Layout a plan for them and utilize as little of their time as possible.After all, they are doing you a favor by working additional time than they normally would. As a goodwill gesture, consider offering your co-worker a gift after you recover or better yet, offer to switch places with them during their sick leave.Just because you’re ill, doesn’t me an you can’t make decisions from your home.Create direct reports and ensure you setup a procedure ahead of time to train one of your colleagues on the most high-priority tasks that need to be done even during your absence. Being prepared beforehand can save you a bunch of last minute requests that your coworkers may not be able to fulfill.4. Keep Yourself Available Through Phone and EmailUnfortunately, being sick doesn’t pause real life.This means that your clients are bound to be surprised to not find you in your usual workspace and would undoubtedly be ticked off that you didn’t notify them beforehand.Hence, it’s necessary to keep an open line of communication even if you’ve taken a sick leave to ensure operations are running smoothly.If you’re sick and are unable to attend the phone, ask a family friend or member to write a formal email on your behalf and forward it to all your contacts in one go.At the very least, you’ve managed to notify them and there won’t be any awkward confrontations the next time you’re back in the office.A wonderful tip is to create drafts for various reasons in your office emails and store them beforehand. When the time comes you can simply select the appropriately filled draft and hit send to all your clients and this saves you time and effort.If you’re able to take phone calls, ensure you create a priority list with the high-priority tasks at the top and the low priority ones at the bottom.You can start calling the most important clients and explaining your situation to postpone important events and meetings.You can take a short break after you’ve gone through the most important calls of the day and conduct the low-priority ones after you’re well rested. In this way, you can still manage to work while being sick and increase your efficiency by 50%.5. Maintain a Doctors’ Note as a Sick Leave PracticeDoctors’ notes are valuable pieces of information that can enable you to prove to your boss that you were indeed sick and were suffering from a severe ailment. It’s important to receive medical care and ensure that you request a note from the doctor to ensure your boss fully understands that you aren’t staging a magnificent performance.Unknown to many employees is the fact that when you sign the contract for a new job, you’re missing an important instruction in the written decorum of the company. Any employer can request a doctor’s note to validate your sick leave and while many managers might not really need to, it’s always worth noting down that when requested, it’s within your obligatory right to provide them with one.If you feel you are being unjustly scrutinized or perhaps your boss is bad mouthing you for taking a sick leave, you can approach the ADA(The Americans with Disabilities Act).The ADA helps employees with a physical or mental condition to claim their right to a sick leave and has established many laws for the benefit of the employees. It’s worth fighting f or your right.AVOID THESE 2 IMPORTANT THINGS WHEN CALLING IN SICK1. Don’t Fake ItFaking a cough or two just to call in sick is something most managers are used to.If you need a day to get away from the hectic responsibilities of your work, come clean and tell your manager about it.You will even be praised and be given the much-needed day or two off. Staging an act that could very well win you an Oscar is something that is a turn off to your team and your managers.Don’t pretend to overdo a cough on the phone when calling in sick, there’s nothing that says FAKE in big black bold letters even if you’re genuinely sick.Unless you’ve been tasked to do a formal call-in, stick to emails to mention your status. You can utilize one of the many pre-created drafts to send over to your boss and team while providing you with several minutes of valuable rest time.Here a few things to keep in mind when making a call or sending an email about your sickness â€"Keep the Conversation Short an d PoliteYou don’t need to prove to your boss that you’re sick while on phone, the tone says it all.Simply demonstrate your gratitude to your boss and appreciate the understanding they have in you.This is how a professional usually gets a satisfactory sick leave without further questions. Don’t go overboard explaining your condition as this plays no role at the moment and can be communicated after your illness.E-mail or Phone? Which is the better optionIf you’ve got an extremely bad fever and are low on energy levels, a simple draft should suffice to explain your reason for the absence.If you’ve lined up a big project or a critical meeting, then a phone call is a must to convey your instructions on how to phase out the rest of the plan.A practical rule of the thumb is â€" if it’s just another regular day at the office with no priorities in line, choose email. For everything else, make the call. Don’t ever send a text, this is considered extremely rude and unprofessional .Mondays and Fridays may seem the most suspicious days to take a leaveWhile being sick is not something you’ve got a control over, the days that you pick as your sick leave can seem like you’re taking an extended vacation.2. Don’t Use Your Sick Days as Vacation Leave and Refrain from Silly ExcusesSick days are valuable resources for every professional and they have been allotted by companies to ensure their employees get time off to recover.Using sick leaves to extend a weekend into a long leave of absence is unacceptable. The worst that could happen is when you indeed fall sick, there won’t be any left to make use of and in such an event it can cause you a great deal of anxiety.Here are some of the excuses that you should never call upon when calling in sick â€"The Hangover DramaA hangover isn’t a sickness, it’s a self-imposed condition caused by your inability to control last night’s drinking spree.Hence, it’s on you and not on the company if you suffer from the ef fects of a long party the night before.The simplistic rule to follow here is to consider your off days and save one day as backup such as Sunday to recover from a bad hangover.In short, choose Friday or Saturday for long parties and use Sunday as a day to recover so you’re ready to tackle the infamous Monday without an excuse.The Weather is Everything but SunnySevere weather is another common excuse that employees tend to throw around to ensure they get a paid sick leave.Unless there is a typhoon, hurricane, or blizzard ravaging across your city and the roads are closed off, there’s absolutely no reason to take a precious day off from work.If there is a severe weather alert, your company is bound to consider it as a major health hazard and call an emergency day off by shooting out emails to all the team members. You don’t need to decide on it.Need to Recharge my Mental HealthUnless you’re the employee of the year and you are practically burnt out from working day in and day out, you aren’t getting past your manager with this excuse.In fact, you will lay claim to the fact that you aren’t a serious employee to take into consideration when promoting and you’ll lose all the respect earned from your team members.Recharging your mental health is just another term for “vacation leave” and should be used only during your vacation package. However, if you’ve recently lost a loved one, it’s understandable to take a day or two off from work to grieve, most companies let their employees off the hook without further questions.SHOULD YOU CONSIDER WORKING FROM HOME INSTEAD OF COMING IN?Many bosses usually frown upon the act of an employee calling in sick. To minimize any negative feeling towards you, you can always choose to work at home if your body is able to. Although it’s extremely important to get adequate rest to ensure you don’t prolong your sickness and in turn extending your sick leave.According to the Centers for Disease Control and Preven tion, you’ve got about a 20% chance of falling sick in a year.Considering that’s a 2 in 10 chances that you will experience a sick day in a year, you’re better off conducting yourself from home when you are able to. It’s not always advisable to arm yourself with cough drops and head on down to the office and infect others and cause a massive crisis-like situation to the company that you work for.Times like these call for the presence of mind â€" How do you work without being in the office?Complete offline tasks such as creating reports and address your clients and make cold calls from the comfort of your home. Your office isn’t necessarily your workplace, your home can convert as a makeshift office during your sick days.By working from home, you show your selfless dedication to your manager and team and show that you don’t like taking sick days if you aren’t committed to solving the challenges of the company. This also motivates you to be efficient always, including in your sickness.Although your physical being may be suffering from the constant loss of energy, your mental balance can stay as focused as if you were at your office.Try training yourself to work during a sickness on a voluntary basis and you can assure yourself that you will be able to win the respect of your peers as well as understand the term “being efficient even in your sleep”.A QUICK CHECK LIST TO FOLLOW WHEN CALLING IN SICKHere’s a quick checklist that you can print and carry around with you or pin up to ensure you don’t forget the things you should be doing when you’re sick. You could also add your own custom bullet points to the list.Call/Email your team and managers at the break of dawn or at the start of the dayEnsure all your clients are being redirected to your phone and emailSave drafts beforehand explaining why you can’t make it to the officeFix a doctor’s appointment and ensure you request a doctor’s noteFollow-up with your trusted colleague at differ ent points of the dayKeep your conversation as limited as possible when conversing with your co-workers by phoneComplete tasks as best as you can from home but only if you’re able toWhen in doubt between rest and work â€" Choose the former and get better quickHere’s a useful infographic to consider when you are confused whether to call in sick or not.CONCLUSIONAnd before you are done asking the most important question, the answer is â€" Yes! You can get fired for repeated faults on faking sickness. So, don’t for one moment utilize the sick leave for a grand vacation in Rome, you may end up jobless if your employer finds out and the FMLA (Family and Medical Leave ACT,) will not be able to protect you from regaining your old job back.There you are the grand rules to follow when committing yourself to a sick leave and it’s truly a disastrous experience to be sick, but we wish you better days and a happy working environment in all the days to come!

Friday, May 22, 2020

Why Bank Robberies Have Increased Since 1994 - 919 Words

I found an article that conducted a study to find why bank robberies have increased since 1994. The focus of the study was focused to the Chicago Police Department and the Chicago Office of the Federal Bureau of Investigations (FBI) where the use of statistics of bank robbers, interviewing bank robbers, and determining what kind of security measures and security equipment was been used, the gathering of the information was used to gain insight into bank robbery (Carrol Loch, (1997). The study first focused on basic information of bank robberies, by using records that the FBI kept when investing bank robbery scenes. This information was very helpful in determining the trends of bank robbers; the investigators gave suggestions to institutions on how to create better security measures. The investigator took all the FBI reports and placed them into a database for future reference (Carrol Loch, (1997). The investigator wanted to learn what motivated bank robbers so they interviewed offenders either in federal or local correctional institutes. They have currently interviewed five offenders, varying from robbers who passed notes to armed single robbers to a group or gang robberies. They found that robbers would avoid certain banks do to security systems; investigator also learned how offenders planned robberies. They found that all offenders interviewed would be deterred from robbing a bank if there were uniformed security present. The offenders were aware of the dye packs andShow MoreRelatedWhite Collar Street Crime Essay1198 Words   |  5 Pages(Merriam-Webster). There are many types of crime but the two this paper will focus on are street crime and white- collar crime. There is no definitive definition of street crime, but it is generally thought of as crime that occurs in public such as theft, robbery, car theft, arson, drug dealing, and vandalism. 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Saturday, May 9, 2020

Continuous Personal and Professional Development - 2181 Words

The following essay is a reflection on the role of the teacher in the life-long learning sector and evaluation of my role in it, and secondly the importance of engaging with continuous professional development and a comparison of theories and models of reflective practice and how they can be applied to my own personal development as a learner. The process I have adopted throughout my teaching is a five stage teacher/training cycle, that of identifying needs and planning, designing, assessing, evaluating and facilitating lessons (McGuigan: 2011), whether it is in conjunction with a scheme of work and its corresponding lesson plans, or whether the course and lessons are self-generated. This process allows teachers to handrail good practice†¦show more content†¦It is an excellent tool for eradicating the isolation some teachers may feel. Following on from the above is mentoring. Despite my relative inexperience in teaching in the lifelong learning sector, I work in an environment where I am now one of the more experienced. Mentoring has allowed me to share my ideas and watch others implement them. Their periodic return for advice reassures me that I am acting as a role model as well as helping new instructors acclimatise backed up with the confidence that they are not alone as the learn the curriculum, not to mention helping me improve as a mentor. The 7th role is as an institutional leader, a role which involves sitting on committees. For me this is by default as I am required to attend weekly and monthly conferences and analyse the information before passing it on to my department. This role allows me to be given the vision of my institution and along with how other people interpret it, informs me better on how my teaching and that of my peers is best directed to fulfill that vision. In the role as an ICT coach, I have been able to share thoughts with fellow teachers on how best to incorporate the wide range of I.T available as well as pamphlets and resources which may otherwise have been neglected. This is a two way process as when regular consultation is carried out, I was finding that new initiatives were being createdShow MoreRelatedThe Concept and Importance of Continuous Professional Development (Cpd)1724 Words   |  7 PagesThe concept and importance of continuous Professional Development (CPD) 1) Concept of CPD: CPD in other words known as Continuous Development, this is because the development of professional people increasing day by day and there is no end in 21st century. Due to Global competition the number of professionals increasing day by day, Clients is ever more aware of their rights and the levels of quality that they demand are continuously rising. 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Wednesday, May 6, 2020

BE Reading Free Essays

This involves creating the opportunity, space and time needed to think about practice and the appropriate action emerging from a reflective thinking process. We argue that being a ‘thoughtful agent’ alls requires a deeper understanding of self and of the nature of personal engagement with ongoing reflective activity. This approach enables restrictions to question the ‘paradigms in which one is operating’ (Peters and Vandenberg, 2011 : 63) and to be responsive to the need for change and quality improvement in relation to the specific needs of spellbinder, families and settings. We will write a custom essay sample on BE Reading or any similar topic only for you Order Now Consequently, it requires an understanding of what we mean by being a reflective practitioner, including understanding the terminology we use and the interpretation we apply throughout this chapter. Table 4. 1 explains how we use the terminology that surrounds reflective practice in this chapter. Reflective practice has been identified by educators as beneficial for quality improvement (Arises and Chon, 1978; Bout et al. , 1985; Brookfield, 1987; Broadband and McGill, 2007). It has been described as a generic term for ‘those intellectual and affective activities in which individuals engage to explore their experiences in order to lee ad to new understandings and appreciation’ (Bout et al. , 1985: 19). In addition, reflection has the capacity to create ‘alternative and more productive ways of organizing the workplace’ (Brookfield, 1987: 14). Together these two statements indicate that examining our actions and activities, both at a cognitive and an emotional level, can help restrictions to think and learn from experience in order to improve practice. Such reflective activity can also be creative, offering different, new and more effective ways of organizing things, whether applied when working with children or colleagues or to the way we organism the environment. Expressed simply, the core principles Of reflective practice involve reflective thinking and learning, which are used to inform decisions and actions in practice, and by implication, improve quality. A number of ‘models’ have evolved to support reflective thinking and practice. Many of these, such as Kola’s (1984) model of experiential learning, Ghee and Ghee’s (1998) ‘reflection-on-practice’ and Brookfield (1995) ‘lenses’, have the clear purpose of supporting critical thinking about experience and using what is learnt from this process 60 to inform future actions. In addition Ghee (2011: 28) draws on the work of Bandmaster (1991 ) and asks us to see reflection as a mainstreaming process that includes the satisfaction of four personal needs of purpose, value, efficacy and self-worth’. However, while many recognize the role of self- reflection and the influence of a range of personal ‘drivers’, they do not serially encourage practitioners to understand, take ownership or utilities the unique nature Of their reflective activity. Ownership draws on a range Of personal factors, such as heritage, disposition, skills and understanding. A deeper level of engagement with reflective activity also requires understanding and appreciation of personal potential. Self-awareness can support reflective practice that is personally meaningful and therefore more likely TA produce the energy and drive necessary to make significant differences in terms of quality. This perspective includes recognition and acceptance of unique ways of being reflective and how this is supported by an individual’s specific professional qualities. Such an approach values different ways of engaging with reflective activity and professes no single model or particular professional context. It also supports the development of reflexive practitioners who question ‘taken for granted beliefs’ and develop an ‘understanding that knowledge is contestable’ (Peters and Vanderbilt, 201 1: 63). Peters and Vanderbilt argue that such reflexivity supports a focus on ‘doing the right things rather than doing things right’, a key principle hat we believe underpins the process of improving quality. An individual’s reflective activity often takes place within dynamic and changeable socio- cultural context, which shapes the processes, responses and individuals involved. While the core values and principles of an individual al may remain constant and be articulated and understood as a basis for reflective activity, there are many ways of responding to issues according TA context. Developing as a reflective practitioner means being someone who is able to act in ways that make a qualitative difference and it requires an understanding of the current socio-cultural context and how this affects the nature Of professional responses. Brotherliness’s (1986) ecological model may help us to explore this concept of socio-cultural influence on reflective identity and practice. According to Frontbencher an individual’s development is affected by a series of environmental influences: the ‘mortises’ of family, school, or neighborhood; the ‘ecosystem’ of a town, local policy, or economic influences; and the ‘Microsystems’ of cultural influences, national policy, or pervading ideology. A practitioner’s reflective reactive may likewise be influenced by colleagues, peers, managers and parents at a setting; who in turn may be influenced by local quality improvement policy, REFLECTIVE PRACTICE 15 THE KEY TO QUALITY IMPROVEMENT 61 risk awareness, and economic status; and overall this is influenced by central government policy and perhaps the perceived ‘culture’ of the type of setting. Therefore practitioners may subtly shift in perceived identity and consequent reflective responses according to the social and environmental situation in which they find themselves. The ability to engage positively and constructively thin a changing professional landscape is supported by an individual’s understanding of both that landscape and what is possible within a particular situation in terms of their personal responses and qualities. Just as external socio-cultural spheres influence responses, the reflective activity by an individual may influence future qua a lit y improvement in others because the practitioner is an ‘active’ agent within their professional context. Recognizing and valuing the impact of this agency may offer an opportunity for reflective practice to be a ‘means of empowerment, leading to change at the individual ND societal level’ (Cable and Miller, 2008: 173). Developing a strong sense of one’s own identity as a reflective practitioner can have a significant impact on both individual and collective confidence to engage in reflective activity as a means of improving quality. Reflective practice as a ‘way of being’ Understanding reflective practice as a ;way of being’ that is owned and experienced by a practitioner encourages the development of an individual as a ‘reflective professional practitioner rather than as a technician’ (Moss, 2008: xiii). This allows for the identification of different ways of engaging within a recess. A ‘technician’ may go through’ the motions of making changes in practice by following a prescribed model of reflective practice. However, it IS essential for a ‘reflective professional practitioner’ to emotionally and intellectually ‘own’ the process (Moss, 2008: xiii). Ownership means acknowledging that reflective practice can include the use of deeply embedded intuitive ‘reflex responses’ and ‘ways Of knowing’ (Atkinson and Clayton, 2000: 2). Atkinson and Clayton argue that we should value ‘other forms of reflection’ that do not focus solely on reason and articulation; rather, unconscious insight draws on the whole of what has been known’; the enormity and complexity of which cannot always be articulated (2000: 5). Encouraging practitioners to use their full range of personal resources within reflective activity is essential. It is possible that compliance with a prescribed ‘model’ limits reflective potential by indicating one preferred way of proceeding towards 62 reflection, or even towards quality improvement. We would suggest that without alternatives, such reliance on an external ‘expert’ model may leave practitioners feeling De-skilled and disemboweled. Recognition of reflective practice as unique to individuals celebrates difference, recognizes personal development and is therefore inclusive. Enabling practitioners to utilities their full range of personal resources within reflective activity requires a critical view of what is involved. There is a view that intuitive forms of knowledge and ‘ways of knowing’ have been unjustly ignored in our rational technical world (Atkinson and Clayton, 2000). For Atkinson and Clayton intuitive and ‘tacit’ forms of knowledge in practice are of equal value and should be equally validated and respected. They even argue that there are times when we can ‘think too much’ in rationalizing processes when we should rely on a more instinctive way of being. This suggests that there is a form of professional reflection that is much more intuitive and instinctive and relies on the inner resources of a practitioner. We see this as important in the context of developing early years practice, which requires an understanding of many complex issues. Kernel and Sheep (2010) suggest that reflective intuition should be respected as a ‘way of knowing’ that is particularly useful in dealing with complexity. Intuitive reflective practice respects and releases inner qualities and understandings, which inform actions taken to improve quality in practice. Many models of reflective practice represent what seems to be a relatively simple process. Investigation into the nature of a practitioner’s ‘real life’ participation in reflective practice reveals a complex array of professional qualities applied and synthesized in different ways at different times according to the situation. Understanding the coming together of the individual al and context offers a way of understanding reflective activity from a deeply arsenal perspective. Through a process of making ‘human sense’ (Donaldson, 1987) of one’s own reflective activity, practitioners Gin evaluate the ways and extent to which they make changes for the better in all aspects of life. Personalized reflective activity that becomes a positive experience and rewards aspects of self is more likely to become a disposition or ‘habitat mind’ (Arnold, 2003), owned by the individual. Practitioners who understand the nature of their own engagement in reflective practice are more likely to be . Emotionally as well as intellectually involved in the process. How to cite BE Reading, Papers

Tuesday, April 28, 2020

Little Women a Look Into Gender Roles in 1941 free essay sample

A look into gender roles in 1941 The photograph Little Girls with Their Dolls and Buggies (1941), taken by Russell Lee, is of two young girls pushing baby dolls in carriages along a sidewalk in Caldwell, Idaho. Its a bright, sunny day and the girls are walking alone, with no other people visible in the photograph. Both girls are smiling, and the girl pictured to the right is looking intently at the girl to the left. Behind the girls to the left we see a respectably sized house, and well maintained lawn. Behind them, the sidewalk endlessly continues to trees in the background. To the right of the picture there is a road which contains no cars. It appears to be in a middle to upper class, suburban neighborhood. They appear to be approximately the same age and are similarly dressed. It is an intriguing image that demonstrates traditionally accepted feminine gender roles during the mid 20th century. We will write a custom essay sample on Little Women: a Look Into Gender Roles in 1941 or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Gender roles can typically be defined as the behaviors and attitudes expected of male and female members of a society by that society. In 1941, women were typically expected to stay home and raise a family. They were also supposed to be social and they were Judged quite often on their appearance. These expectations of women were pushed upon them at a young age, as shown through the photograph. The girls appear to be about seven years old and they are already pushing around baby dolls and exploring their maternal roles. How identical the girls look is also a very revealing. The girls dont look to be related, but yet they appear as mirror images of one another. They both have short, groomed hair, pulled away from their face. They are both wearing dresses, and are pushing ractically identical carriages. They are shaped to look how a woman should and follow the same expected role of women during that time period. I think its also important to note the variety of social influences of gender roles. Influence and reinforcement of gender roles often comes from the toys children play with. Dolls are traditionally a toy for girls. Dolls tend to represent feminine traits and help to prepare young girls for the task of motherhood. Children tend to get most of their toys from a parent or family member, who often has the strongest influences on a ersons gender role. The young girls clothing also comes from their parents. This is yet another example of how their parents reinforce their perceived gender roles. Peers also play a role in shaping ones anticipated gender role. During this time especially, peers responded more and got along better with children who fit traditional gender roles. This again leads back to how similarly the girls are dressed in the photograph and the similar toys theyre playing with. The girls are being conditioned to follow society and their peers assumed ideas of the roles they are expected to play. The girls are also walking alone; their parents are not in visible in the photograph. Its like their parents are trying to let them be little adults. Theyre preparing their children for their expected future of raising a family. Imposed gender roles are often hard to escape. Especially during the 1940s, women had very specific assumed roles. While looking at Russells photo, you may see two young girls, message about traditional gender roles. Its a shame that these girls, at such a young age, were being shaped to a very limited role that society has deemed to be their future.

Friday, March 20, 2020

Romeos Tragic Flaw essays

Romeos Tragic Flaw essays In the play Romeo and Juliet, Romeo is the tragic hero who through his tragic flaws, ended the life of his bride and his own. In Shakespeare plays, tragedy is identified as a story that ends unhappily due to the fall of the protagonist. Romeos act of falling in love too quickly and deeply, pride, and poor decision making are his tragic flaws and contributes to the death of Juliet and himself. By analyzing Romeos tragic flaws, it is evident that the play is classified as a tragedy. One of Romeos tragic flaws, being that he falls in love too quickly and too deeply brings him to an awful end. In the beginning of the play we are introduced to Romeo being deeply and hopelessly in love with Rosaline. However, Rosaline did not feel the same way about Romeo. Romeo shows he is unhappy with Rosalines actions when he says to Benvolio: Not having that which makes having short. This very well illustrates how deeply Romeo was in love with Rosaline. In the next scene we realize that Romeo has fallen for another, which is Juliet. We can quickly identify Romeos flaw of falling in love too quickly and deeply when he forgets about his feelings for Rosaline and concentrates on Juliet. Juliet too realizes that Romeos love for her was too fast when she said: It is too rash, too undivisd, too sudden. Romeos strong love for Juliet made him act without thinking when he kept contemplating suicide and saying that he cant live without her. He shows he his love for her when he sees she her dead, not knowing it was a plan, then drinking the poison right away. He shows he will do anything for his new love, including risking getting caught by the Capulet guards at the orchard, and killing himself to be with Juliet. This tragic flaw of Romeo was the reason ...

Tuesday, March 3, 2020

What to Do in Budapest 11 Top Attractions

What to Do in Budapest 11 Top Attractions SAT / ACT Prep Online Guides and Tips One of the rising stars in European tourism, Budapest’s charms and sights are gaining popularity worldwide. Gone are the days when the Hungarian capital was widely thought of as a drab administrative city, filled only with aging Communist-era buildings. These days, Budapest tourism is rapidly growing and savvy visitors know that it's one of Europe’s great cities with diverse architecture, unique cultural experiences, and some of the most inventive nightlife on the continent. Are you wondering what to do in Budapest? Follow this guide to immerse yourself in Budapest’s history, traditions, and culture. By visiting the Budapest attractions listed below, you’ll find yourself relaxing in opulent thermal baths, paying your respects at moving WWII memorials, admiring the largest Parliament in Europe, and drinking a new cocktail in the gardens of a formerly abandoned warehouse. This list of 11 things to do in Budapest is organized into four groups, based on the location of the sites: Castle Hill, City Park, Parliament and Around, and Central Pest. This organization lets you visit sites near each other, instead of spending your trip running from one end of town to the other. Many people choose to stay in downtown Pest because it has the most accommodation options, but Budapest has a great subway system to help you get from one area to another. You can buy individual subway tickets, day passes, or multi-day passes. I'd recommend that you spend at least a half-day to a full day in each area, and, if you choose to stay longer, you’ll find Budapest has enough activities to fill any itinerary. The Top 11 Places to Visit in Budapest Check out this map of the best things to do in Budapest and then read more about each below. This map can also be viewed online. Castle Hill - Red Matthias Church Fisherman’s Bastion Buda Castle City Park - Blue Szà ©chenyi Bath Vajdahunyad Castle Heroes’ Square Parliament and Around - Purple Hungarian Parliament Building Shoes on the Danube Monument Central Pest - Green Hungarian State Opera House Ruin Pubs Great Synagogue Castle Hill Attractions You can find some of the most famous- and stunning- things to see in Budapest in the Castle Hill area, including gorgeous architecture and historically important sites. Matthias Church Matthias Church is one of the top Budapest attractions, and it’s a center point of Castle Hill. Built in the late 13th century, Matthias Church was the first parish church in Budapest, and it has remained an important symbol of the city. During its long history, Matthias Church hosted multiple royal coronations, was damaged by cannon fire, and spent over 100 years as a Mosque while the city was under Ottoman rule. Even if you feel like you’ve seen enough European churches to last a lifetime, stop for a look at Matthias Church. It doesn’t have the soaring grandeur of larger churches like Notre Dame and Milan’s Duomo, but its unique colors and design make it one of the most pleasant churches in Europe to view. The church was extensively restored in the 1900s, and today it’s popular with tourists who come to see and photograph the Gothic architecture, stained glass windows, and vibrant diamond-patterned roof tiles. The church is also home to the Ecclesiastical Art Museum. The museum (which charges a small fee) begins in the church’s medieval crypt and continues to the chapel. While touring the museum, visitors can view the tombs of 10th century rulers King Bà ©la III and his wife Agnes, replicas of the royal Hungarian jewels, and assorted other jewels and religious objects. Fisherman’s Bastion One of the most unusual sights in Budapest, Fisherman’s Bastion is an elegant, neo-Gothic terrace overlooking the Danube. The site was named for a group of fisherman who defended the city from invasion during the Middle Ages. Walking under gleaming white arches and along staircases and terraces, visitors get sweeping views of the Danube, including a birds-eye view of Parliament. The seven turrets of Fisherman’s Bastion represent the seven Magyar tribe leaders who are regarded as the founders of modern-day Hungary. It may not seem like there’s much to do at Fisherman’s Bastion since the site is little more than an attractive lookout, but many tourists spend far longer than they planned here, strolling from one end of the terrace to the other and taking rolls of photos. When visiting Castle Hill, make Fisherman’s Bastion your first stop (easy to do since it’s very close to public transportation stops). The weather in Budapest can be fickle, and there are times when the terrace and its views are enveloped by fog. It usually clears later in the day, so check back periodically if the weather isn’t cooperating when you first stop by. Fisherman’s Bastion is located next to Matthias Church, and together these two sites make up one the best places to visit in Budapest. Here you can walk along cobblestone streets, look at historic buildings, and grab lunch in one of the numerous traditional cafà ©s that line the streets. Buda Castle Rising up from the southern end of Castle Hill is the imposing, sprawling structure of Buda Castle. The first castle on this site was completed in 1265, and the castle continued to be expanded and rebuilt throughout the centuries. Buda Castle has been a royal residence for generations of Hungarian rulers, besieged by Ottoman armies, taken over by the Habsburgs, occupied by Nazis during WWII, and designated as a World Heritage Site in 1987. Today, the grounds are adorned with numerous statues and gardens, which visitors can admire while walking around the castle. Within the castle are a trio of museums and institutions: the Budapest History Museum, the Hungarian National Gallery, and the National Szà ©chà ©nyi Library (the museum and gallery both require tickets, but the library is free to visit). Each site is well worth a visit, focusing on the history of the city, Hungarian art, and a collection of rare books and paper, respectively. If you can only visit one museum (visiting all three can make for a long day), my pick is the Budapest History Museum. Far more than a dry collection of artifacts, this museum spans three floors and includes such varied exhibits as recreations of opulent rooms as they looked when used by royals, paintings by Hungarian Masters, and excavated rooms of the medieval castle in the basement. There are no cars allowed on Castle Hill unless you live or work in the area, and the lack of noise and traffic contributes to the feeling that you’ve stepped into the past and are far removed from the bustle of the city down below. City Park Attractions City Park is a large public park that includes some of Budapest's best attractions. Szà ©chenyi Bath Visiting the city's famed baths is one of the top things to do in Budapest. Budapest’s abundant thermal waters means there are numerous baths to choose from, but, in terms of history and enjoyability, Szà ©chenyi Bath is far and away your best option. As the largest thermal bath in Europe, Szà ©chenyi Bath is one of Budapest’s iconic sites. When it was built in 1913, the architecture followed the Neo-baroque style popular at the time, and it’s easy to feel like you’re wading through the private pool of a royal palace as you move between the bath’s 18 different pools. When you arrive, you’ll be given a waterproof key card to a personal closet-sized locker, where you can change into your swimsuit (required) and store your belongings while you’re in the baths. There are both indoor and outdoor pools to choose between in Szà ©chenyi. While the indoor pools have a wider variety of temperatures, as well as saunas and steam rooms, most people prefer the outdoor baths because they are typically brighter and cleaner, and they let you enjoy the cheery, slightly aged exterior of the baths. Szà ©chenyi is popular with tourists, and there will likely be a number of people soaking alongside you, but it remains an institution for Budapest locals. They come here to relax after work, socialize, and play a game of chess on one of the waterproof boards in the baths. At Szà ©chenyi, you can also purchase additional services, such as massages. Ticket prices vary depending on the time of day you visit and if you want a cabin or locker, but expect to pay about 5000 Florin (about $18 USD). Massages are an additional 5000-35000 Florin. The thermal waters keep temperatures warm year-round at Szà ©chenyi, so both the indoor and outdoor baths remain open in winter. Vajdahunyad Castle Vajdahunyad Castle is located close to the Szà ©chenyi Baths within City Park, but many people breeze past this tucked away corner of town without pausing to explore. Vajdahunyad can’t compare to Buda Castle’s immense size or history, but this attractive and somewhat mysterious castle earns major points for charm. The castle was originally built in 1896 as a temporary structure of cardboard and wood to celebrate the 1000th anniversary of the Hungarian Conquest of the Carpathian Basin. However, Budapest’s citizens were so taken with it that the present structure, made of stone and painted in muted blues, purples, and oranges, was soon constructed. Set back amongst trees and gardens, the castle’s shadowy exterior reveals features from numerous architectural styles that showcase the variety and evolution of architecture in Budapest. Additional touches to the castle include sunken windows, a portcullis, and numerous turrets overlooking a boating lake turned ice skating rink in the winter. A popular spot for photos is the brooding, slightly creepy statue of Anonymus, a mysterious Hungarian notary and chronicler who lived around 1200 AD. Inside the castle is Museum of Hungarian Agriculture (small fee charged), but even if you’re not interested in the museum’s focus, it’s pleasant simply to walk through the castle’s interior and climb to the top of the two tallest towers for views of the park. Vajdahunyad Castle was only built at the turn of the 20th century, but its historical architecture and romantic setting make it feel far older. A short stroll around the castle or a picnic on its grounds beside the lake is an excellent way to take a break from a busy day of sightseeing. Heroes’ Square Another attraction near City Park, Heroes’ Square does not take long to visit, but it’s one of the city’s most important landmarks. Located at the end of Andrssy Avenue, Heroes’ Square is well-worth visiting to get a sense of Hungary’s history. Built in 1896 to celebrate Hungary’s 1000th anniversary, Heroes’ Square is the largest square in Budapest. The focal point of the square is tall column adorned with a statue of the archangel Gabriel. Flanking the base are seven statues representing the seven original Magyar chieftains. Surrounding the column are 14 pillars with statues of other important Hungarian figures. Viewing the square’s statues and plaques is a great crash-course on the history of the country. Among the statues you’ll see famed Hungarian saints, rulers, and warriors. A plaque in the square dedicates the site "To the memory of the heroes who gave their lives for the freedom of our people and our national independence." During Budapest’s long history, the city was occupied by foreign invaders several times and ruled by a communist regime. Heroes’ Square now serves as a physical monument to Hungary’s dedication to democracy. The square can be visited both during the day and at night, when it is lit up by floodlights. Parliament and Around If you're interested in more recent history, this is the area for you, with monuments to Hungary's tumultuous 20th century. Hungarian Parliament Building The Hungarian Parliament Building is the seat of the National Assembly of Hungary and one of the icons of Budapest. Its design was based on the UK Parliament Building, the Palace of Westminster, but the Parliament of Budapest is no mere copycat. This is one of the oldest and largest legislative buildings in Europe, and since the day it was completed in 1904, it has been the largest building in Hungary. Built along the bank of the Danube River, this soaring Gothic Revival building looks surprisingly delicate up close, with numerous intricate details. Visitors can opt to take a guided tour of the building to see sights including the House Chambers, the inside of the dome, the grand entrance staircase, the Hungarian crown jewels, and a small museum. Tickets, which are 5200 Florin for non-EU citizens, must be purchased beforehand, either online or in person. Make sure you view the Parliament Building both during the day and at night, when the entire structure is lit up, its reflection glowing in the river. Shoes on the Danube Bank Memorial There are World War II memorials far grander than this collection of 60 pairs of iron shoes scattered along the edge of the Danube, but that doesn’t make this site any less moving. The monument was conceived by director Can Togay and created by sculptor Gyula Pauer to honor the people murdered by soldiers of the Arrow Cross, a national socialist party in Hungary during WWII. Between 1944 and 1945, an unknown number of victims, mostly Budapest Jews, were taken to the edge of the Danube and shot, their bodies falling into the river. Before they were killed, they were told to remove their shoes, which could be resold for a profit. This small memorial, a few hundred feet from Parliament, poignantly pays tribute to the victims. The shoes were designed to accurately represent shoes from that period, and they were created in many different styles to represent the wide variety of victims: men, women, business people, teenagers, and children. The shoes are often adorned with ribbons or flowers left as tokens of remembrance by visitors. Walking along the memorial and viewing the empty shoes strewn forlornly along the Danube’s bank is a powerful reminder of the lives lost in WWII. Central Pest Attractions Central Pest is where many visitors choose to stay, because it offers easy access to all the Budapest sights. It's also home to some of the city's most exciting cultural attractions. Hungarian State Opera House Housed in an elegant neo-Renaissance building dating from 1884, the Hungarian State Opera House is a point of pride for many Hungarians, yet it’s much less well-known than many other European opera houses. For a small fee, you can take one of the regular tours of the opera house. After passing the figures of two of Hungary’s most renowned composers in the entrance, you’ll enter a foyer with marble columns and vaulted ceilings. Like all grand opera houses, this one has a sweeping staircase dominating the entrance, used by Hungarian’s past elite to see and be seen. Other opulent touches include a brass chandelier and vast murals decorating the walls and ceilings. The theater itself is decorated with red velvet and gold leaf. It seats over 2,600, with the royal box in the center of the horseshoe-shaped rows. Many people simply opt for a tour, but the Hungarian State Opera House is one of the best places to view an opera in Europe, particularly if you’ve never been to one before. The opera house often performs crowd pleasers like Carmen and Madama Butterfly, which are a great introduction for opera novices. Additionally, tickets here are significantly cheaper than at most other opera houses; you can often get a seat for less than $10 USD. At many other opera houses, tickets this price are typically for the standing area only, and standing shoulder to shoulder with other guests for several hours is enough to fatigue even opera aficionados. At the Hungarian Opera House, you’ll be able to enjoy the opera in comfort without breaking the bank. In addition to operas, the building also hosts ballets and other performances. The Hungarian State Opera House website has a calendar showing all upcoming events. Operas are subtitled in both Hungarian and English, and opera season lasts from September to July. Ruin Pubs Budapest can be heavy with history, so for a change of pace and to get a sense of how the city enjoys itself, head to one of Budapest’s many ruin pubs, drinking establishments that have sprung up in recently un-abandoned buildings. Rumor has it that ruin pubs first came into existence in 2001, out of a need for a cheap place to drink. No two ruin pubs are alike, but all of them share several characteristics: they’re housed in a formerly abandoned building, the furniture is eclectic and often pre-used, and they show their links to the community by hosting local bands or DJs or having local artists decorate the interior. Most of the ruin pubs are located in Budapest’s old Jewish Quarter, in the streets behind the Great Synagogue. In a ruin pub, you could find yourself in a cozy former home with couches for seating, taking to the dance floor in a large, rambling warehouse, tasting plinka (a traditional fruit brandy) under the glow of old-fashioned lanterns, enjoying beers in a courtyard filled with picnic tables, or learning Hungarian phrases at one of the regular informal classes the pubs host. One of the best aspects of ruin pubs is that you never know what type of experience you’re going to get until you walk inside. The best ruin pub to start at is the original, Szimpla Kert. Occupying a large, formerly abandoned building, Szimpla is filled with mismatched and often antique furniture and decorations. Outside there’s a spacious courtyard which includes an old Trabant car guests can lounge in. Ruin pubs attract a mostly young, international crowd, but you’ll find all types here. Local Hungarians often frequent them, as do older visitors. Ruin pubs may be most enjoyable to visit on a warm summer evening, but they’re open year-round, with outdoor spaces typically closed in the winter. Great Synagogue This massive building, also known as the Dohny Street Synagogue, is the largest synagogue in Europe and the second-largest in the world. It was completed in 1859 in the Neo-Moorish style, giving it a distinct look compared to many other synagogues. Inside the Great Synagogue is seating for more than 3,000 people, as well as the Jewish Museum, which has exhibits on Jewish traditions and the history of Judaism in Budapest. The building is rich with history. During World War II, the site was bombed by Nazi planes, and it later became one border of Budapest’s Jewish ghetto. In the grounds surrounding the synagogue is a graveyard where over 2,000 people who died in the Jewish ghetto during the winter of 1944-1945 are buried. There's also the Heroes’ Temple honoring Hungarian Jews who died during World War I and the Holocaust Memorial. The Holocaust Memorial, also known as the Emanuel Tree, is particularly striking to see. Crafted by Hungarian sculptor Imre Varga, the memorial takes the form of a delicate and intricate weeping willow. The name of a Hungarian Jew killed during the Holocaust has been engraved on each metal leaf. In my opinion, this is one of the world’s most beautiful and moving Holocaust memorials. If you don’t have time to tour the synagogue, you can get a view of the tree through the gate surrounding the grounds. You can take a self-guided tour around the synagogue, but a guided tour (available at the entrance) can help you navigate the large site and fully appreciate its historical importance. Entrance to the synagogue costs 3000 Florin and includes access to both the building and its grounds.

Sunday, February 16, 2020

The intersection between ethics and politics in ANIMAL RIGHTS about Essay

The intersection between ethics and politics in ANIMAL RIGHTS about Animal Genetic Testing - Essay Example Animal testing is one of the most ethically gray points in society. One can say that it can is a necessary evil as it is an important factor in keeping technology, as in medical technology. We need to know how certain chemicals can work in our body when dispensed, hence animal testing. In short, the development of drugs is one of the reasons why there is animal testing in the midst. However, we are also aware of the fact that animal testing is brutal as it is (most of the time) done without painkillers – meaning, the animals would suffer for the plight of the people, plight of the people that make them miserable and take their services for granted, as they are inferior – animals as they are called. There are about 20 million animals that are killed every year for experiments. 75% of the said figure are killed because of medical testing and that the rest of them are killed to try new products (as in animal testing). Ten percent of the 20 million figure are said to be und ergoing procedure without any pain killers. There are many people, especially the animal rights advocates, who oppose the use of animals in medicine and health services, that is true. They are imposing various government to give restrictions on companies and businesses that do not respect animal rights for research. However, this movement is also taking a call to stop as there are also a growing number of people who do not want to stop experimenting on the animals. This is due to the fact - or fear – that medical research and technology would stop growing at the rate that it is growing now. This means that the stoppage of the use of animals in research would curtail the growth of the knowledge and scientific progress. Pain is an intrinsic evil. Those people who argue that painful experimentation on animals should be avoided, or stopped (in a perfect world), say that, and anything (or anyone) that causes pain to another creature or animal is immoral. Using the words of Jeremy Bentham, one cannot say (or use the argument) that the animals â€Å"can’t talk, or reason† hence they can be used. The real question that should be asked is â€Å"do animals suffer?† The answer is yes. They do suffer because they feel pain. The scientist who conducts research the rat for some new formulation about a new drug knows that the rat feels pain because it needs to know how the human would react if it is subjected to the new drug – will the new drug cause pain to the patient or not? Every writhe and struggle of the anilam is recorded and then will be used to improve and design a new drug. Even the fact that the scientist will have to let the animal choose to what its setup is: to have electric shocks or to have hunger, is immoral. Although the data will be used and collected to know whether ulcers of some kind would be formed, the doctor still uses the animal to know how the human mind works as the rat and humans have the same neural responses. Basically, we are like rats. Pain is an intrinsic evil. It does not matter if it is experienced by a man, child or animal. If it is wrong to inflict pain on a human being, then it is wrong to inflict pain on an animal, or any creature for that matter. Another thing that should be

Sunday, February 2, 2020

Lesson Plan Reflections Essay Example | Topics and Well Written Essays - 500 words

Lesson Plan Reflections - Essay Example The activities I planned supported the learning objective since they were related to the lesson work and aimed at enabling the student understand the concepts for every lesson. For example, reading the story on time and pointing to the clock to depict the different time mentioned engaged the students since it entailed use of both audio and visual learning aides. This fostered the students to meet the learning objectives. The other activity for the math lesson on time around the world required the students to draw picture to indicate digital times as well as analog time. This meets the objective, which was to enable students to tell time using analog and digital clocks. I could tell whether the students were successful in achieving the objectives depending on how they performed in the after lesson assessments. For example, the objective of the Social Studies lesson on Girl on the Go was to enable students remember the different countries they had learnt in the history lesson. The story used different monuments in association with different countries. To assess if the students met the objectives, I asked post-reading questions that covered what happened in the beginning middle and end of the story. Moreover, I asked the students to mention the countries that Isabella visited, what the different countries were famous for, and they answered correctly. Additionally, the students were supposed to tell each other countries they had visited, to see if they remembered the previous lessons. I realized that student learn better through activities. For example, after reading the story about the Girl on the Go, I realized that students could easily remember the courtiers mentioned in the story as compared to other countries covered in previous lessons. They would also associate different monuments with the countries where they are located. Moreover, use of pictures and a clock in the math class made the lesson interesting and the students were excited when they realized

Saturday, January 25, 2020

Expression and Purification of recombinant Green Fluorescent Protein

Expression and Purification of recombinant Green Fluorescent Protein ABSTRACT: The purpose of this experiment was to determine if a His-6 tagged recombinant form of Green Fluorescent Protein could be expressed in a pRSETA vector of E. Coli. This was determined through multiple procedures beginning with purifying the sample with Ni +2 agarose chromatography which showcased the relative fluorescent activity of the samples, which elution sample two (E2) had approximately 100,592.2 RFU/mg . The yield of total protein was found by use of a Bradford Assay and a standard curve. The purity of the GFP was determined by comparing the intensity of bands that appeared at around 31.4 kDa (the molecular weight of rGFP) to a molecular weight ladder on an SDS-PAGE gel. The Western Blot test, utilizing a nitrocellulous membrane, confirmed the expression of rGFP. The Western Blot confirmed that the correct bands were analyzed in the SDS-PAGE gel which E3 had an estimated purity of 0.4, indicating a yield of approximately 29.4 ug of rGFP for the third Elution (E3) after a total p rotein amount of 49 ug was extrapolated from the Bradford assay. INTRODUCTION: The Green Fluorescent Protein was first isolated from the Aequorea Victoria jellyfish and studied by Osamu Shimomura. In other organisms other than this specific jellyfish, there are fluorescent proteins with more color varieties than just green (Ward). GFP is able to fluoresce due to the formation of a chromophore in its center that resides in a stable beta barrel structure. The interactions between Ser64 and Gly67 on an alpha strand form a five member ring structure and its this structure that contains properties for fluorescence. When ultraviolet light is absorbed at 395nm, an emission wavelength of 510nm is seen as fluorescent green light. The excitation wavelength of 395nm excites the electrons within the protein and raises the energy of the protein. As the protein loses energy, it emits the energy at a wavelength of 510nm. Wild type GFP does not have immensely visible fluorescence, therefore a genetically modified form of GFP, GFPuv, was used in order to better observe fluoresc ent activity. For rGFP to be fully expressed, GFPuvs open reading frame was pRSETA vector, which was cut by a restriction enzyme at sites that complimented the start and stop codons of the His-6 tag sequence. A T7 promoter and Xpress Epitope were also genetically added into the vector with the purpose of simplifying purification. T7 polymerase minds to the T7 promoter and activates the production of GFP, while the Xpress Epitope allows for the binding of a primary antibody. The binding of a primary antibody allows for the binding of a secondary antibody which contains horseradish peroxide, producing bands on the nitrocellulose membrane. (2 pg 351) Histidine-6 tags primary purpose is to aid in the purification of rGFP proteins in affinity chromatography. Histidine has an affinity to bind with transition metal ions, and is why a Nickel+2 agarose chromatography was chosen. When a sample is passed through the Nickel+2 agarose column, the His-6 tags fused with rGFP protein will bind to the column and any proteins not of interest or containments will pass through the column. To elute the bound rGFP proteins from the column, elution buffer containing Imidazole is used and competes with the histidine residues for the binding sties to the Nickel+2 agarose column due to its higher affinity for them. Elutions from the column therefore contain rGFP. (2 pg 359) The purpose of this experiment was to see if a His-6 tagged recombinant form of GFP, from the E. coli strain BL21pLysS, could be expressed and then purified using Ni+2 agarose affinity chromatography technology. If this experiment is successful, an inexpensive and relatively simple method to measure gene expression and locate and track proteins may be at hand. MATERIALS AND METHODS: Bacterial Expression Grow two bacterial cultures: G, containing BL21, DE3, pLysS, and the plasmid pRSETA-GFPuv with the GFP sequence, and V, which is identical to G, but it does not have the GFP sequence. Incubate both cultures at about 37Â °C until OD600 equals 0.5. Transfer 1ml of each culture into separate centrifuge tubes and centrifuge to obtain a pellet. The supernatant from the tubes will be discarded and then label the tubes V0 and G0 and stored at -20Â °C. Induce the rest of the culture with IPTG and allow to grow for 3 hours. After the 3 hours, collect 1ml of each culture and centrifuge. Label the tubes V3 and G3 and were store at -20Â °C. Collect 15 ml of G and centrifuge, and label it as G3-15ml and store at -20Â °C. (1 pg 105) Preparation of Crude Extract After the slow freeze process, add 1 ml of breaking buffer (10mM Tris, pH 8.0; 150mM NaCl) to the frozen bacterial pellet G3-15ml, and pipette up and down until completely dissolved. Then transfer the contents to a centrifuge tube and vortex for 5 minutes and place in a 37Â °C water bath for 10 minutes. Afterwards, incubate in dry air at 37Â °C. Centrifuge the mixture at 4Â °C and transfer the supernatant into a clean centrifuge tube and take a small sample to label as GCE. The remaining supernatant will go through the Nickel+2 agarose column. (1 pg 110) Ni+2 NTA Chromatography Pack a 3ml plastic syringe with a small amount of glass wool to prepare the Ni+2 agarose column. After that, pipet 1 ml of breaking buffer into the syringe to remove any air bubbles. As the breaking buffer is going through the syringe, pour some breaking buffer into the leur-lock and then screw it onto the syringe. Add 1ml of 50% Ni+2 agarose into the column and then open the leur-lock to pack the agarose matrix in the colum. Add 5 ml of breaking buffer subsequently into the column to push the ethanol of the Ni+2 agarose through the column. Then close the leur-lock and apply the crude extract to the column and allow 5-10 minutes for the histidine tags on the rGFP to bind with the Ni+2 agarose beads. Open the leur-lock and collect the non-crude extract in a centrifuge tube labeled W1 for the first wash. Add 0.5ml aliquots of breaking buffer to the column and collect the washes in tubes W2-W10. Add 10 increments of 0.5ml of elution buffer and collect in tubes E1-E10. Then store your GC E, W1-10, and E1-10 samples at -20Â °C. (1 pg 111-2) Bradford Assay Analysis To create a Bradford assay, mix protein sample with water, and then add Bradford reagent dye. Vortex the mixture and then transfer it to the wells of a microtiter dish, so that the absorbance of your protein sample can be determined at 595nm using a spectrophotometer. To determine the amount of total protein present in the sample volume, you must create a Bradford standard curve using 0, 2.5, 5, 10, 15, and 20 ug of BSA and the absorbance values observed to determine a best-fit line. Perform the assay in singlicate using samples W1-W6 and E1-E6 to determine if the microplate data falls within the standard curve. Once you have determined what volume of sample to use, repeat the Bradford assays two more times for each sample. Use the standard curve and experimental absorbance values to extrapolate the total protein present in the volume of sample you use. (1 pg 124-6) SDS-PAGE/Coomassie Blue Analysis of rGFP Fractions Create a 12% Resolving Gel by mixing water, 30% Acrylamide, 10% APS, TEMED, and 4x resolving buffer [0.75M Tris pH8.8, 0.4%SDS]. Pour the resolving gel into a gel electrophoresis set up and overlay with some water. Allow the resolving gel to polymerize and proceed to making a 5% Stacking gel by mixing water, 30% Acrylamide, 10% APS, TEMED, and 4x stacking buffer. Pour the stacking gel on top of the resolving gel and immediately insert a comb to form the wells. Prepare your loading samples with 4x sample loading buffer and then vortex, boil, and centrifuge. Do this for the G0, G3, GCE, W2, W3, E2, and E3 samples (or fractions that contained the most rGFP fluorescence). (1pg 111-2) After the gel has solidified, transfer the apparatus into the electrophoresis tank and load the electrophoresis buffer into the tank. Then load your samples into lanes one through seven respectively along with a marking ladder to compare the distances the samples traveled after electrophoresis. Electrophorese at 200V for approximately 45 minutes and then stain your gel with Coomassie Blue dye. To remove the stain that has not been absorbed by proteins, the gel is soaked in dilute acid and methanol. Western Blot Develop an SDS-PAGE gel as previously described using your sample fractions and transfer the proteins onto nitrocellulose transfer membrane using two locking cassette lids with sponges and filter paper encompassing the gel and nitrocellulose. Remove the nitrocellulose and stain the membrane with Ponceau S stain, and allow to incubate for approximately 2 minutes. Once stained, wash the membrane with nanopure water until bands are visible on the membrane. Next, perform the blocking step by placing the membrane in a container containing 5% non-fat dry milk/TBS solution and incubate on a shaking platform. Pour out the blocking solution and add 0.05% Tween 20/TBS solution and then allow it to incubate on a shaking platform, and then pour out the solution. Repeat this wash step two more times. Add mouse IgG anti-Xpress epitope MAb and allow incubate on a shaking platform this is the primary probe step. Then repeat the wash step as mentioned previously three more times, and add Sheep IgG a nti-mouse IgG conjugated horse radish peroxidase polyclonal anti-serum solution (the secondary probe). Allow the membrane to incubate, and then perform the wash step twice. For the final wash, use only TBS, and then add TMB substrate solution and incubate until appropriate banding is shown. Stop the reaction with distilled water and scan or photograph the membrane as the results may fade with time. RESULTS: In the specific strain of E. coli, BL21pLysS, pRSET-GFP represses the lac promoter which is part of the operon needed for T7 polymerase activity. T7 polymerase binds to the T7 promotes of pRSET-GFP and activates the His-6 tag that expresses the fluorescence of GFP. Even though some GFP is produced this way, it is experimentally not enough, so IPTG is added to repress the repressor so an increase of GFP production can occur. V0, V3, G0, and G3 were all bacterial cultures of E. coli with specific distinctions between all of them. V0 and G0 were collected from bacterial cultures that were not yet induced, but G0 contained the GFP sequence while V0 did not. V3 and G3 were the V0 and G0 samples after induction had occurred for 3 hours, and again G0 contained the GFP sequence while V0 did not. W1-W10 were washes collected from the Ni+2 agarose column using the breaking buffer whereas E1-E10 were washes collected using elution buffer. Figure 2. SDS-PAGE/Coomassie Blue stained gel analysis of rGFP SDS-PAGE gel, which was used to analyze our purification of rGFP from a crude extract, and the corresponding molecular weight. This gel was made with a 12% resolving gel and a 5% stacking gel. Wildtype rGFP has a molecular weight for 27kDa. The ladder has kDa rungs of 97.4, 66.2, 45.0, 31, 21.5, and 14.4. The band for rGFP has been indicated on the figure. The different samples were taken during our purification. The highest protein containing washes and elutions were used in the SDS-PAGE gel. G0 represents a sample containing rGFP prior to induction. G3 represents a sample containing rGFP after 3 hours of induction. GCE represents the GFP crude extract after the slow-freeze/quick-thaw process. Figure 3. Western Blot of rGFP We used the Western Blot to detect, and more importantly, quantify proteins that react with antibodies. The Ponceau S stain was performed to highlight the existence of our proteins successfully being transferred from the SDS-PAGE gel. We then performed a blocking step with 5% non-fat dry milk/TBS solution followed by washes with 0.05% Tween20/TBS. The probe first used was mouse IgG anti-Xpress epitope MAb solution, which we followed with washes of Tween20/TBS solution. The second probe used was sheep IgG anti-mouse IgG conjugated horseradish peroxidase polyclonal anti-serum solution, which we followed with two washes of Tween 20/TBS solution with the third wash being pure TBS. Then, TMB solution was added to develop the nitrocellulose membrane and this reaction was stopped by pouring distilled water over the membrane. Above are the 8 lanes, G0, G3, GCE, W2, W3, E3, E4, and a known molecular weight ladder, respectively. CONCLUSION/DISCUSSION: A great amount of research has been put into the field of monitoring gene expression and there are currently many research laboratories that use labeled antibodies and other various means to do so. However, this new recombination technology, a vector can be created to provide a simpler and less expensive approach that can even be performed in vivo. The purpose of this experiment was fulfilled as His-6 tagged rGFP from a particular E. coli strain was successfully expressed, purified, qualified, and quantified by means of Ni+2 agarose chromatography. GFP was initially expressed as a histidine tagged protein that was inserted in E. coli and subsequently a crude extract of this protein was isolated. Throughout the experiment, we were able to inspect and measure the fluorescing activity of our elutions with the use of handheld UV lights. GFP was successfully expressed because of the fluorescent activity that was emitted when shown under UV light. This rGFP was purified using a Ni+2 agarose column which allowed for the binding of the His-6 tagged rGFP to the Ni+2 agarose beads in the column, and subsequently let all other proteins not of interest and containments to be collected in washes. Then, rGFP proteins were eluted by passing elution buffer containing Imidazole through the column because Imidazole has a higher affinity for the Ni+2 agarose beads than does Histidine. Therefore, one could expect that most if not all of the rGFP would be found in the elution washes and would present higher fluorescing activity than the washes with breaking buffer. Figure 1 clearly supports this argument. From referencing Figure 1, the most rGFP was found in E2 because it exhibited the highest fluorescent activity in RFUs. Using a standard curve based off of known quantities of mass of a certain protein, one is able to extrapolate the amount of protein present in ones sample. This can be used to estimate the amount of rGFP activity. For instance, E2s specific activity was found to be approximately 100,592.2 RFU/mg. W1-W6 had the least amount of fluorescing activity because the washes were done prior to the addition of Imidazole through the column. This is expected as Imidazole would flush out rGFP in the elutions, which would produce the highest GFP fluorescing activity. Percent purity of the rGFP samples were approximated using the SDS-PAGE gel with Coomassie blue staining to allow visible bands to appear which were used for comparisons. The molecular mass from each individual sample was determined by comparing the bands with the bands of the known weight ladder. The percent purity of each individual band was determined by comparing the bands against each other. The molecular weight of wild type rGFP is 27 kDa, and the molecular weight of rGFP for mutant rGFP used for this experiment was approximately 31.4 kDa with the 40 extra amino acids that were added taken into account. For example, E3 percentage of purity was found to be .4. The amount of protein yielded was 29.4 ug of rGFP for the third Elution fraction (E3) after a total protein amount of 49 ug The Western Blotting technique detected and quantified proteins that reacted with a specific antibody. In our case, it verified rGFP was expressed and whether or not it fell in the range of the expected molecular weight of 31.51 kDa. Lanes GCE, G0, G3, and E3 all contained a bright band around 31.4 kDa, indicating that rGFP was existent. This result corresponds to the expected of E3 having the one of the highest rGFP activity. The fact I had used E3 for the SDS-PAGE and Western Blotting skewed my results, but E2 and E3 had the highest activity for me. Even with my error, GFP was effectively expressed and purified and its finding is important to science because it can be used to track cancer causing cells one day to help figure out where problem spots begin and can help eliminate the issue before it escalates indicating that fluorescent proteins might eventually be clinically useful in cancer patients (Hoffman). This can occur since GFP can successfully be expressed in other organisms as this experiment through the use of E.coli, humans cells can certainly be tagged and label the tumour before treatment and then monitor for fluorescence after treatment to identify possible recurrence or metastases (Hoffman). The same can be said with heart disease and other health related problems. The future of GFP remains limitless when scientists can unlock its full medical potential. REFERENCE/BIBLIOGRAPHY (1) Rippel, Scott. BIOL 3380 Fall 2010: Biochemistry Lab Manual. The University of Texas at Dallas. Richardson, TX. (2) Rippel, Scott. BIOL 3380 Fall 2010: Biochemistry Lab Lecture Notes. The University of Texas at Dallas. Richardson, TX. (3) Ward, William. History of GFP and GFP Antibodies. Brighter Ideas. 2009. 26 Oct, 2010. http://www.brighterideasinc.com/proteins-antibodies/history-of-gfp-and-gfp-antibodies/ (4) Ninfa, Alexander J. and David P. Ballou (1998). Fundamental Laboratory Approaches for Biochemistry and Biotechnology. (5) Hoffman, Robert M. Uses of Fluorescent Proteins to Visualize Cancer In Vivo: Can Fluorescent Proteins be Used in Humans? 2005. 28 Oct. 2010. http://www.medscape.com/viewarticle/513975_6